Tilling by sequencing.
نویسندگان
چکیده
TILLING is a method to find mutations in a gene of interest by scanning amplicons from a mutagenized population for sequence changes, commonly a single nucleotide. In the past 5 years, mutation detection by sequencing has become increasingly popular. This chapter details the experimental flow for TILLING-by-Sequencing, highlighting the critical steps involved in tridimensional pooling of genomic DNA templates, preparation of libraries for high-throughput sequencing, and bioinformatic processing of the sequence data.
منابع مشابه
TILLING and associated technologies.
As part of a collaboration between the John Innes Centre (JIC), Norwich, UK and the Centre for Signal Transduction and Metabolomics (C-STM) at the Institute of Botany (IOB), the Chinese Academy of Sciences, Beijing, a workshop on ‘TILLING and Associated Technologies’ was held at the IOB on 15–16 April 2010. Through sponsorship from the UK Biotechnology and Biological Sciences Research Council (...
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عنوان ژورنال:
- Methods in molecular biology
دوره 1284 شماره
صفحات -
تاریخ انتشار 2015